三氮菌素 C
Triacsin C (WS 1228A) 是一种口服有效的、胞内长链酰基辅酶 A 合成酶 (ACSL) 抑制剂，可从金黄色链霉菌 (Streptomyces aureofaciens) 中分离得到。Triacsin C 通过抑制 ACSL 来抑制 TAG 积聚成脂滴 (LD) 。Triacsin C 可有效抑制轮状病毒复制。
茯苓新酸 B
Poricoic acid B 是一种可以从茯苓中分离得到的三萜类化合物。Poricoic acid B 抑制细胞内的脂质蓄积，并降低肝细胞损伤标志物水平。Poricoic acid B 可抑制 NO、TNF-α、IL-1β 和 IL-6 的生成，进而发挥抗炎活性。Poricoic acid B 可用于代谢功能障碍相关脂肪性肝病和炎症疾病的研究。
甘草异黄酮 B
Licoisoflavone B 是一种被发现存在于甘草中的具有口服活性的黄酮类化合物。Licoisoflavone B 通过靶向 SCD1 的脂质代谢重编程以及抑制 Th17/IL-17 介导的炎症来缓解银屑病。Licoisoflavone B 可抑制超氧阴离子的生成以及超氧阴离子诱导的脂质过氧化。Licoisoflavone B 可与拉沙病毒核蛋白紧密结合，可用作拉沙病毒的核蛋白拮抗剂。Licoisoflavone B 对致癌诱变剂具有抗诱变活性，可通过阻止 DNA 损伤发挥作用。Licoisoflavone B 可用于银屑病、拉沙热、炎症及癌症的研究。
Floramanoside F 是一种黄酮醇苷类化合物。Floramanoside F 与 1 型糖尿病肾病 (T1DN) 的关键靶点酶 (Fasn、Cyp2e1、Cyp4a32) 具有强结合力，能抑制脂质堆积和氧化应激，从而减轻肾脏炎症与纤维化。Floramanoside F 具有中等强度的自由基清除作用，SC₅₀ 为 25.1 μM。Floramanoside F 可用研究 1 型糖尿病肾病及糖尿病并发症。
莱苞迪苷D
Rebaudioside D 是一种口服有效的甜味剂，靶向激活 FXR、调节 Acetyl-CoA Carboxylase 和抑制 3-hydroxy-3-methylglutaryl-CoA reductase。Rebaudioside D 调控胆汁酸稳态与脂质代谢，降低脂肪酸与胆固醇的合成速率，具有抗脂肪生成、保肝、抗脂肪变性、调节肠道菌群、增强次级胆汁酸代谢、抗内毒素、调控胆汁酸转运及抑制胆汁酸外排等多重作用。Rebaudioside D 还减少体重增长、内脏脂肪堆积、肝脏甘油三酯与胆固醇蓄积、肝脏脂质过氧化，并降低循环中脂多糖结合蛋白的水平。Rebaudioside D 还增强肠道细菌次级胆汁酸代谢通路，上调回肠有机溶质转运蛋白 α 的基因表达，下调肝脏胆盐输出泵蛋白的基因表达。Rebaudioside D 不影响葡萄糖稳态、改变总热量摄入或粪便能量排泄、诱导体重增加、加重肥胖、促进肝脏脂肪变性、损伤棕色脂肪组织功能，也不会改变骨骼肌代谢相关蛋白。Rebaudioside D 可用于饮食诱导肥胖及肥胖相关研究。
Tibesaikosaponin V 是一种三萜双糖苷，可从北柴胡 (Bupleurum chinense DC) 的根的甲醇提取物中分离得到。Tibesaikosaponin V (TKV) 是一种三萜双糖苷，可从Bupleurum chinense DC 根的甲醇提取物中分离得到。Tibesaikosaponin V 抑制脂质积累和甘油三酯含量的发生，对脂肪细胞没有细胞毒性。Tibesaikosaponin V 抑制核转录因子的 mRNA 表达，例如过氧化物酶体增殖物激活受体 γ (PPARγ) 和 CCAAT/增强子结合蛋白 α (C/EBPα)。Tibesaikosaponin V 抑制 3T3-L1 前脂肪细胞分化。Tibesaikosaponin V 可用于肥胖及其相关代谢紊乱的研究。
Malabaricone C 是一种具有口服活性的非竞争性鞘磷脂合成酶 (SMS) 抑制剂，对 SMS 1 和 SMS 2 的 IC50 值分别为 3 μM 和 1.5 μM。Malabaricone C 降低小鼠体重增加，提高葡萄糖耐量，减少肝脏脂质积累，对高脂肪饮食诱导的脂肪肝有显著的预防作用。Malabaricone C 具有抗炎作用，发现于 Myristica cinnamomea King 的果实中。Malabaricone C 有望用于肥胖和由 T 细胞过度激活而引起的免疫性疾病的研究。
番茄皂苷A
Esculeoside A 是一种具有口服活性的螺甾烷型甾体生物碱糖苷，存在于 Lycopersicon esculentum var. cerasiforme 和 Lycopersicon esculentum 的成熟果实中。Esculeoside A 是一种心脏保护剂和降糖剂。Esculeoside A 可调控心脏组织中的 NF-κB、Nrf2/Keap1 信号通路，减轻炎症反应与凋亡 (apoptosis)，阻止心肌细胞肥大，并降低血清脂质水平。Esculeoside A 可通过调控 IRS-1、GCK、AMPK 和 PEPCK 的表达来调节糖代谢，降低空腹血糖，改善葡萄糖耐量。Esculeoside A 可抑制 TLR4/p-NFκB 信号通路，从而阻碍树突状细胞成熟及异体 T 细胞增殖，并抑制乳腺癌与黑色素瘤细胞的生长。Esculeoside A 可抑制 ACAT 活性，减少巨噬细胞中胆固醇酯的积累。Esculeoside A 可用于糖尿病心肌病、2 型糖尿病、特应性皮炎、肿瘤及动脉粥样硬化性疾病的研究。
地骨皮甲素
Kukoamine A 是一种具有口服活性可穿透血脑屏障的精胺类生物碱，被发现存在于宁夏枸杞 (Lycium chinense，L. chinense) Miller 的根皮中。Kukoamine A 可抑制纯化的簇生锥虫锥虫硫醇还原酶和大豆脂氧合酶 (lipoxygenase)，并激活 μ-阿片 (μ-opioid) 受体。Kukoamine A 能够抑制癌细胞增殖、迁移和侵袭，引起 G0/G1 期细胞周期阻滞并诱导细胞凋亡 (apoptosis)。Kukoamine A 具有神经保护作用，还可诱导自噬 (autophagy)。Kukoamine A 可抑制 LPS (HY-D1056) 诱导的 NO、ROS、PGE2、TNF-α、IL-1β、IL-6 的产生以及 COX-2 的活性。Kukoamine A 可通过下调 Srebp-1c 逆转棕榈酸诱导胰岛素抵抗、脂质蓄积和氧化应激。Kukoamine A 可用于癌症、感染、炎症、代谢性疾病和神经系统疾病的研究，例如胶质母细胞瘤和帕金森病。
三氮菌素 C
Triacsin C (WS 1228A) 是一种口服有效的、胞内长链酰基辅酶 A 合成酶 (ACSL) 抑制剂，可从金黄色链霉菌 (Streptomyces aureofaciens) 中分离得到。Triacsin C 通过抑制 ACSL 来抑制 TAG 积聚成脂滴 (LD) 。Triacsin C 可有效抑制轮状病毒复制。
Malabaricone C 是一种具有口服活性的非竞争性鞘磷脂合成酶 (SMS) 抑制剂，对 SMS 1 和 SMS 2 的 IC50 值分别为 3 μM 和 1.5 μM。Malabaricone C 降低小鼠体重增加，提高葡萄糖耐量，减少肝脏脂质积累，对高脂肪饮食诱导的脂肪肝有显著的预防作用。Malabaricone C 具有抗炎作用，发现于 Myristica cinnamomea King 的果实中。Malabaricone C 有望用于肥胖和由 T 细胞过度激活而引起的免疫性疾病的研究。
莱苞迪苷D
Rebaudioside D 是一种口服有效的甜味剂，靶向激活 FXR、调节 Acetyl-CoA Carboxylase 和抑制 3-hydroxy-3-methylglutaryl-CoA reductase。Rebaudioside D 调控胆汁酸稳态与脂质代谢，降低脂肪酸与胆固醇的合成速率，具有抗脂肪生成、保肝、抗脂肪变性、调节肠道菌群、增强次级胆汁酸代谢、抗内毒素、调控胆汁酸转运及抑制胆汁酸外排等多重作用。Rebaudioside D 还减少体重增长、内脏脂肪堆积、肝脏甘油三酯与胆固醇蓄积、肝脏脂质过氧化，并降低循环中脂多糖结合蛋白的水平。Rebaudioside D 还增强肠道细菌次级胆汁酸代谢通路，上调回肠有机溶质转运蛋白 α 的基因表达，下调肝脏胆盐输出泵蛋白的基因表达。Rebaudioside D 不影响葡萄糖稳态、改变总热量摄入或粪便能量排泄、诱导体重增加、加重肥胖、促进肝脏脂肪变性、损伤棕色脂肪组织功能，也不会改变骨骼肌代谢相关蛋白。Rebaudioside D 可用于饮食诱导肥胖及肥胖相关研究。
地骨皮甲素
Kukoamine A 是一种具有口服活性可穿透血脑屏障的精胺类生物碱，被发现存在于宁夏枸杞 (Lycium chinense，L. chinense) Miller 的根皮中。Kukoamine A 可抑制纯化的簇生锥虫锥虫硫醇还原酶和大豆脂氧合酶 (lipoxygenase)，并激活 μ-阿片 (μ-opioid) 受体。Kukoamine A 能够抑制癌细胞增殖、迁移和侵袭，引起 G0/G1 期细胞周期阻滞并诱导细胞凋亡 (apoptosis)。Kukoamine A 具有神经保护作用，还可诱导自噬 (autophagy)。Kukoamine A 可抑制 LPS (HY-D1056) 诱导的 NO、ROS、PGE2、TNF-α、IL-1β、IL-6 的产生以及 COX-2 的活性。Kukoamine A 可通过下调 Srebp-1c 逆转棕榈酸诱导胰岛素抵抗、脂质蓄积和氧化应激。Kukoamine A 可用于癌症、感染、炎症、代谢性疾病和神经系统疾病的研究，例如胶质母细胞瘤和帕金森病。
茯苓新酸 B
Poricoic acid B 是一种可以从茯苓中分离得到的三萜类化合物。Poricoic acid B 抑制细胞内的脂质蓄积，并降低肝细胞损伤标志物水平。Poricoic acid B 可抑制 NO、TNF-α、IL-1β 和 IL-6 的生成，进而发挥抗炎活性。Poricoic acid B 可用于代谢功能障碍相关脂肪性肝病和炎症疾病的研究。
甘草异黄酮 B
Licoisoflavone B 是一种被发现存在于甘草中的具有口服活性的黄酮类化合物。Licoisoflavone B 通过靶向 SCD1 的脂质代谢重编程以及抑制 Th17/IL-17 介导的炎症来缓解银屑病。Licoisoflavone B 可抑制超氧阴离子的生成以及超氧阴离子诱导的脂质过氧化。Licoisoflavone B 可与拉沙病毒核蛋白紧密结合，可用作拉沙病毒的核蛋白拮抗剂。Licoisoflavone B 对致癌诱变剂具有抗诱变活性，可通过阻止 DNA 损伤发挥作用。Licoisoflavone B 可用于银屑病、拉沙热、炎症及癌症的研究。
Tibesaikosaponin V 是一种三萜双糖苷，可从北柴胡 (Bupleurum chinense DC) 的根的甲醇提取物中分离得到。Tibesaikosaponin V (TKV) 是一种三萜双糖苷，可从Bupleurum chinense DC 根的甲醇提取物中分离得到。Tibesaikosaponin V 抑制脂质积累和甘油三酯含量的发生，对脂肪细胞没有细胞毒性。Tibesaikosaponin V 抑制核转录因子的 mRNA 表达，例如过氧化物酶体增殖物激活受体 γ (PPARγ) 和 CCAAT/增强子结合蛋白 α (C/EBPα)。Tibesaikosaponin V 抑制 3T3-L1 前脂肪细胞分化。Tibesaikosaponin V 可用于肥胖及其相关代谢紊乱的研究。
Floramanoside F 是一种黄酮醇苷类化合物。Floramanoside F 与 1 型糖尿病肾病 (T1DN) 的关键靶点酶 (Fasn、Cyp2e1、Cyp4a32) 具有强结合力，能抑制脂质堆积和氧化应激，从而减轻肾脏炎症与纤维化。Floramanoside F 具有中等强度的自由基清除作用，SC₅₀ 为 25.1 μM。Floramanoside F 可用研究 1 型糖尿病肾病及糖尿病并发症。
番茄皂苷A
Esculeoside A 是一种具有口服活性的螺甾烷型甾体生物碱糖苷，存在于 Lycopersicon esculentum var. cerasiforme 和 Lycopersicon esculentum 的成熟果实中。Esculeoside A 是一种心脏保护剂和降糖剂。Esculeoside A 可调控心脏组织中的 NF-κB、Nrf2/Keap1 信号通路，减轻炎症反应与凋亡 (apoptosis)，阻止心肌细胞肥大，并降低血清脂质水平。Esculeoside A 可通过调控 IRS-1、GCK、AMPK 和 PEPCK 的表达来调节糖代谢，降低空腹血糖，改善葡萄糖耐量。Esculeoside A 可抑制 TLR4/p-NFκB 信号通路，从而阻碍树突状细胞成熟及异体 T 细胞增殖，并抑制乳腺癌与黑色素瘤细胞的生长。Esculeoside A 可抑制 ACAT 活性，减少巨噬细胞中胆固醇酯的积累。Esculeoside A 可用于糖尿病心肌病、2 型糖尿病、特应性皮炎、肿瘤及动脉粥样硬化性疾病的研究。
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
MedchemExpress Validation 03
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
MedchemExpress Validation 04
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
